Academic Activities

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TBSI Greater Bay Area Intellectual Forum Lecture 51【Nanshan i-Park】

  1. 报告主题:Antagonism between the Master Regulators of Differentiation Ensures the Discreteness and Robustness of Cell Fates
  2. 报告人:Dr. Lan MU
  3. 主持:

Time

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September 20th, Thursday, 2018  2:00-3:00 p.m.


Abstract

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The discreteness of cell fates is an inherent and fundamental feature of multicellular organisms. Here we show that cross-antagonistic mechanisms of actions of MyoD and PPARg, which are the master regulators of muscle and adipose differentiation, respectively, confer robustness to the integrity of cell differentiation. Simultaneous expression of MyoD and PPARg in mesenchymal stem/stromal cells led to the generation of a mixture of multinucle- ated myotubes and lipid-filled adipocytes. Interest- ingly, hybrid cells (i.e., lipid-filled myotubes) were not generated, suggesting that these differentiation programs are mutually exclusive. Mechanistically, although exogenously expressed MyoD was rapidly degraded in adipocytes through ubiquitin-protea- some pathways, exogenously expressed PPARg was not downregulated in myotubes. In PPARg- expressing myotubes, PPARg-dependent histone hyperacetylation was inhibited in a subset of adipo- genic gene loci, including that of C/EBPa, an essen- tial effector of PPARg. Thus, the cross-repressive interactions between MyoD- and PPARg-induced differentiation programs ensure discrete cell-fate decisions.


Speaker's Bio

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The majority of my research experience has been focused on understanding the molecular mechanism of skeletal muscle stem cells, as well as their self-renewal, proliferation and differentiation. My previous education and research experience provided me with a strong background in stem cell biology. As an undergraduate student, I mastered some theoretical knowledge of stem cell biology. As a graduate student, in the area of muscle development, aimed to understand how extracellular gene is involved in muscle stem cell’s self-renewal, proliferation and differentiation, and found that semaphorin-3A, an axon guidance protein, secreted form muscle stem cells and promotes their proliferation and differentiation capability by regulation of their master regulator genes expression. Currently, as a postdoctoral fellow in the Asakura lab at University of Minnesota, I am working on the direct lineage reprogramming of mouse embryonic fibroblasts(MEFs) into myogenic cells. The repair of skeletal muscle needs a sufficient number of myogenic cells to replace the damaged tissue, therefore, expansion of cells is generally required. Myogenic cells derived by direct-reprogramming may provide a solution to the limitations of current stem cell-based therapy of muscular dystrophy. Throughout the course of my research experience, I served as the primary or co-investigator in all of my studies. Based on my previous work, I have a strong understanding of the fundamental concepts of muscular stem cells. My dedication and experience in stem cell biology makes me a strong candidate for this application and would allow me to continue contributing to this field of research.


Registration

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Professors and students of TBSI are welcome to attend. The lecture is also open to the public. For off-campus personnel, please scan the QR code and and fill in your information (name, company, contact number, ID number). The language of the lecture is English.